Bio-One Inc. has ranked number 381 in Entrepreneur's 43rd annual Franchise 500. This is the sixth year in a row that Bio-One has been included in the ranking.

A total of 1,177 companies met the requirements, and each franchise was given a cumulative score based on an analysis of more than 150 data points. The 500 franchises with the highest cumulative scores become the Franchise 500 in ranking order. 

"We're grateful to be recognized by Entrepreneur for the sixth year in a row," said Brian Brinegar, president of Bio-One Inc. "The ranking is a direct reflection of our growth, strength of our franchisees, and our ability to consistently deliver care and compassion to our clients."

Succeeding 12 years of franchising, Bio-One has over 115 offices in more than 40 states with services that include crime and trauma scene cleaning, hoarding remediation, odor removal, sewage backups, and more. In 2021, Bio-One also ranked number one in Entrepreneur's Best of the Best for Crime-Scene Cleaning. 

About Bio-One Inc.

Bio-One provides decontamination and biohazard cleanup services while treating clients with the privacy and compassion that difficult moments demand. Bio-One services include suicide cleanup, homicide cleanup, hoarding remediation, junk removal, deceased animal recovery, feces removal and more. Bio-One was first created in 2006 and began franchising in 2010. In 2021, Bio-One joined the Five Star Franchising portfolio. Five Star Franchising is a conglomerate of franchisor brands with a mission to ensure its brands are top performers in technology, marketing, branding and people.